The exon5\7 sequences of the ABO gene were amplified by PCR and sequenced bidirectionally. Blood Institute Background/Case Studies: A recent publication reported that reddish cell transfusions from previously pregnant female donors markedly improved the mortality of transfused male individuals. The findings wouldif truehave much\reaching medical and administrative implications in the management of the blood supply. However, these results may have been affected by biases launched in the statistical analysis. Study Design/Method: We separately analyzed data from three linked blood donor and recipient cohorts including data from the US and Scandinavia, over long time periods. Patients were adopted from the time of 1st reddish cell transfusion for the event of F-TCF both in\hospital and long\term mortality. We used independent Cox regression models to estimate the associations between quantity of reddish cell transfusions from a female donor, a previously pregnant donor, and a donor sex\discordant with the recipientall treated as time\dependentand risk of death while controlling for total number of reddish cell transfusions received using a stratified Cox model. Analyses were performed for overall effect and stratified by recipient sex and age. Results/Getting: We included a total of 53,890 individuals (5,654 deaths), 93,724 individuals (8,519 deaths) and 918,996 individuals (198,537 deaths with longer follow up) in cohorts I, II and III, respectively. There was no association between any of the donor characteristics and in\hospital mortality in any of the three cohorts (Table). Risk ratios per transfused unit from a parous female donor were all non\significant, ranging from 0.99 to 1 1.02. Results were WAY-100635 related for the effect of donor sex and sex\discordance on in\hospital mortality (Table), as well as with long\term mortality in two of the cohorts (data not shown). Effect estimations did not differ with recipient sex, and/or age. Categorical analyses did not display negative effects in greatly revealed individuals. Conclusion: With this joint analysis of data from three large cohorts of transfused individuals, we found no evidence of an association between donor sex, or parity and either in\hospital or long\term patient survival. These null WAY-100635 findings using a related statistical approach across more than a million individuals from heterogeneous medical settings in different countries show that prior findings seem unlikely to reflect true biological effects. (P1\MN1\6) Risk of in\hospital death, in relation to transfusion exposures, for the three cohorts. This study examined the relationship between perioperative RBC transfusions and post\operative VTE within 30 days of a surgery treatment in children ( 18 years). Study Design/Method: Using the pediatric database of the American College of Surgeons National Surgical Quality Improvement System (PEDS ACS\NSQIP) (2012\2014), risk\modified results for VTE (deep venous thrombosis (DVT(/pulmonary embolism(PE)) of pediatric individuals ( 18 years) undergoing elective/urgent/emergent surgeries were compared. Univariate followed by multivariable logistic regression was performed. Results/Getting: N=183,233 children [39,211 babies ( 1 year); 7,857 neonates ( 28 days)] were evaluated. Of these 73.18% underwent elective, 10.03% urgent and 16.80% emergent methods. Commonest surgery types were: general surgery 38.62%, orthopedic 19.68%, urologic 11.51%, otolaryngological 11.02% and neurosurgical 8.66%. About 1.1% (n=1956) children [n=1129 (2.9%) babies; n=507 (6.45%) WAY-100635 neonates] received pre\operative transfusions (within 48 hours of surgery). Six percent (n=11,003) children [n=3,462 (8.83%) babies; n=1,101, (14.01%) neonates] received RBC transfusions intraoperatively (start of surgery until 72 hrs post\op). Transfusions were in response to intra/post\operative bleeding. 197 children (0.11%) [(n=74 (0.2%) babies; n=28 (0.36%) neonates)] had post\operative VTE (including 10 (0.11%) instances of PE). Intra/post\operative RBC transfusions were associated with 1.8\fold higher risk of VTE (modified odds ratio [adjOR]=1.81;95%CI=1.25\2.61), p 0.001] after accounting for various putative risk factors (Table 1). The association was stronger in babies [adjOR?=?3.2; 95%CI?=?(1.88\5.43), p 0.001] and neonates [adjOR?=?5.66; 95%CI?=?(2.30\13.93), p 0.001]. (P5\MN1\6) Pre\operative RBC transfusions were independently associated with post\operative VTE in all children [adjOR]?=?2.30; 95%CI=1.43\3.67), p 0.01], babies [adjOR?=?2.55; 95%CI?=?(1.34\5.43), p 0.01] and neonates [adjOR?=?3.63; 95%CI?=?(1.36\9.67), p 0.05]. Summary: With this prospective registry study of 180,000 children undergoing surgeries, peri\operative RBC transfusions were associated with higher risk modified odds of post\operative VTE. The relationship is definitely also seen in subgroup analysis in babies and neonates. Should these findings be validated inside a prospective establishing, peri\operative pediatric patient blood management strategies need to be explored in these individuals to optimize peri\operative transfusions in WAY-100635 children. P6\MN1\6 Safety Analysis.