The majority of human peripheral gamma delta T cells express the V gamma 9 gene in combination with the V delta 2 gene. features of IL-2-responsive gamma delta T cells. Out of TSPAN9 30 individuals studied, nine were identified as IL-2-responders and three as IL-2-hyperresponders. The TCR V(D)J gene usage from IL-2 stimulated peripheral blood lymphocytes of these IL-2-(hyper)responsive individuals was analysed. The results showed that in most individuals gamma delta T cells polyclonally expanded after stimulation with IL-2. In two IL-2-hyperresponder individuals, however, a monoclonal expansion of a particular V gamma 9+V delta 2+ gamma delta T cell was found. In one of these individuals, this V gamma 9+V AZD2281 manufacturer delta 2+ T-cell clone expressed a very rare gamma delta TCR type because of the presence of an Ala within the junctional region at a conserved position relative to V delta framework residues (delta 97), which is very infrequently used by peripheral blood V gamma 9+V delta 2+ cells. This particular clonotype could also be detected in unstimulated PBL samples taken from that individual, and made up for 30% of the total peripheral gamma delta T-cell pool. These data indicate that in general IL-2-responsive V gamma 9+V delta 2+ gamma delta T cells represent a polyclonal population, reflecting in vivo stimulation with multiple antigens or superantigens. In contrast, monoclonal expansions AZD2281 manufacturer of gamma delta T cells after stimulation with IL-2 can also occur, which may be related to an in vivo stimulation by one particular antigen, rendering this gamma delta AZD2281 manufacturer T-cell type dominant in the peripheral blood. Full text Full text AZD2281 manufacturer is available as a scanned copy of the original print version. Get a printable copy (PDF file) of the complete article (1.3M), or click on a page AZD2281 manufacturer image below to browse page by page. Links to PubMed are also available for Selected References.? 385 386 387 388 389 390 391 ? Images in this article Figure 3 br / on p.389 Click on the image to see a larger version. Selected.